P32. Self-modelling factor analysis combined with analysis of the signal derivative for separation of incompletely separated chromatographic peaks

Yuri A. Kalambet1, Sergey Maltsev1, Yuri P. Kozmin2

1Ampersand Ltd., Moscow, Russia

2Shemyakin Institute of Bioorganic Chemistry, Moscow, Russia

Factor analysis is widely used for separation of incompletely separated chromatographic peaks. We combined this technique with analysis of the angle derivative of the multi-channel chromatogram to get a better choice of basic spectra. Improvement in algorithm is demonstrated by examples.