The 2010 prohibited list of the World Anti-Doping Agency (WADA) covered nine classes of substances (S1-–S9), three categories of prohibited methods (M1–M3) and two groups of substances (P1 and P2) prohibited in particular sports disciplines. All prohibited substances and methods of doping are subject of doping controls.

The constantly increasing numbers of compounds that potentially risk the integrity of sport the complexity of objects and problems in doping control also requires modern multivariate approach for data analysis. Combinations of various chemometric techniques with mass spectrometry should give possibility for efficient investigations in different areas of doping control such as

• Metabolites identification (The tool where the identified metabolic differences observed can be visualized);
• Structure classification (The tool which will help maximize the separation between classes);
• Target analysis;
• Non-target analysis.

Examples of the activities of various chemometric models, illustrating achievements in doping control will be shown. The aims and application problems of multivariate analysis with mass spectrometry for drug testing in doping control successfully, and the variants to accomplish this, will be outlined.